The main topic of this thesis is B2B marketing in the public sector and the role and the importance of improving business operations through the use of e-commerce. Skilfull management of any kind of organization, including public sector, contributes to the full use of resources with the purpose of satisfying interests and achieving objectives of the organization. The most important resource of any organization are people. However, not enough attention has been given to the management of this resource in practice. The main purpose of using e-commerce is to change this state of affairs and, through a detailed study of resource management in the public sector, to enhance the quality of work performance and management, and consequently improve the position of workers in the society as a whole. Managing B2B marketing is a stage in operational management through which the members of the management create the prerequisites for a high level of satisfaction of the resources employed. Developed on the example of the public sector, this thesis presents essential facts and presupositions for the efficiency of B2B marketing in the public sector. The above mentioned topics, which are further developed in this thesis, as well as the expert references based on B2B marketing in the public sector point to the importance of the improvement of business operations and the elements bearing key potential.